A rapid assay for drug sensitivity of glioblastoma stem cells

Hilah Gal; Arik Makovitzki; Ninette Amariglio; Gideon Rechavi; Zvi Ram; David Givol

doi:10.1016/j.bbrc.2007.05.020

A rapid assay for drug sensitivity of glioblastoma stem cells

Hilah Gal, Arik Makovitzki, Ninette Amariglio, Gideon Rechavi, Zvi Ram, David Givol

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Glioblastoma (GBM) is a highly infiltrating, aggressive brain cancer with no available curative treatment. We developed a rapid assay for assessing the effect of various drugs on GBM stem cells. The assay uses a small number of separated CD133+ cells (20,000 in 0.2 ml) in 96-well plate that form neurospheres within 1-2 days. Various drugs disperse the neurospheres within 24-36 h, which can be quantified microscopically. We used the GBM cell line A-172 to develop the conditions for the assay, utilizing Gleevec, the gamma-secretase inhibitor DAPT, and the anti-bacterial peptide amph1D. The results show dispersion of the neurospheres leading to cell death, at relatively low drugs concentrations (<25 μM). Drug combination showed a synergistic effect and disruption of neurospheres under lower concentrations. We applied this assay to the CD133+ cells of surgical specimens from three patients that showed similar results. This assay facilitates a rapid test of drugs on small amounts of fractionated patient's GBM stem cells.

Original language	English
Pages (from-to)	908-913
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	358
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2007.05.020
State	Published - 6 Jul 2007
Externally published	Yes

Bibliographical note

Funding Information:
We thank the Kahn Family Foundation for their support. This research was partially supported by the Wolfson Family Charitable Trust on Tumor Cell Diversity, by the Yad Abraham foundation and by a grant from Ruth & Allen Zeigler for Stem Cell research.

Keywords

96-Well plate
DAPT
Drug sensitivity
Gleevec
Neurospheres

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2007.05.020

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title = "A rapid assay for drug sensitivity of glioblastoma stem cells",

abstract = "Glioblastoma (GBM) is a highly infiltrating, aggressive brain cancer with no available curative treatment. We developed a rapid assay for assessing the effect of various drugs on GBM stem cells. The assay uses a small number of separated CD133+ cells (20,000 in 0.2 ml) in 96-well plate that form neurospheres within 1-2 days. Various drugs disperse the neurospheres within 24-36 h, which can be quantified microscopically. We used the GBM cell line A-172 to develop the conditions for the assay, utilizing Gleevec, the gamma-secretase inhibitor DAPT, and the anti-bacterial peptide amph1D. The results show dispersion of the neurospheres leading to cell death, at relatively low drugs concentrations (<25 μM). Drug combination showed a synergistic effect and disruption of neurospheres under lower concentrations. We applied this assay to the CD133+ cells of surgical specimens from three patients that showed similar results. This assay facilitates a rapid test of drugs on small amounts of fractionated patient's GBM stem cells.",

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AU - Amariglio, Ninette

AU - Rechavi, Gideon

AU - Ram, Zvi

AU - Givol, David

N1 - Funding Information: We thank the Kahn Family Foundation for their support. This research was partially supported by the Wolfson Family Charitable Trust on Tumor Cell Diversity, by the Yad Abraham foundation and by a grant from Ruth & Allen Zeigler for Stem Cell research.

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N2 - Glioblastoma (GBM) is a highly infiltrating, aggressive brain cancer with no available curative treatment. We developed a rapid assay for assessing the effect of various drugs on GBM stem cells. The assay uses a small number of separated CD133+ cells (20,000 in 0.2 ml) in 96-well plate that form neurospheres within 1-2 days. Various drugs disperse the neurospheres within 24-36 h, which can be quantified microscopically. We used the GBM cell line A-172 to develop the conditions for the assay, utilizing Gleevec, the gamma-secretase inhibitor DAPT, and the anti-bacterial peptide amph1D. The results show dispersion of the neurospheres leading to cell death, at relatively low drugs concentrations (<25 μM). Drug combination showed a synergistic effect and disruption of neurospheres under lower concentrations. We applied this assay to the CD133+ cells of surgical specimens from three patients that showed similar results. This assay facilitates a rapid test of drugs on small amounts of fractionated patient's GBM stem cells.

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A rapid assay for drug sensitivity of glioblastoma stem cells

Abstract

Bibliographical note

Keywords

UN SDGs

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