A polymer microstructure array for the formation, culturing, and high throughput drug screening of breast cancer spheroids

Yael Markovitz-Bishitz, Yishay Tauber, Elena Afrimzon, Naomi Zurgil, Maria Sobolev, Yana Shafran, Assaf Deutsch, Steffen Howitz, Mordechai Deutsch

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

Multicellular spheroid models have been recognized as superior to monolayer cell cultures in antitumor drug screening, but their commercial adaptation in the pharmaceutical industry has been delayed, primarily due to technological limitations. The current study presents a new spheroid culture platform that addresses these technical restrictions. The new culturing device is based on a multiwell plate equipped with a glass bottom patterned with an array of UV adhesive microchambers. Each microchamber is designed to accommodate a single spheroid. The system facilitates the simultaneous creation and culturing of a large number of spheroids, as well as screening their response to antitumor drugs. The volume of the spheroids is easily controlled by seeding density. The location of each spheroid is preserved in the same microchamber throughout its growth, treatment with soluble agents, and imaging. The growth ratio parameter, a non-intrusive size analysis of the same spheroid before and after exposure to drugs, was found to be a sensitive indicator for the reaction of MCF7 breast cancer spheroids to cytotoxic drugs. This feature helps reveal the heterogeneity within the spheroid population during the formation process and their drug response, and provides an opportunity to detect specific, highly active or drug-resistant spheroid sub-groups. The advantages of this spheroid-based system make it an efficient drug-screening tool that may be valuable to related fields of research and clinical applications.

Original languageEnglish
Pages (from-to)8436-8444
Number of pages9
JournalBiomaterials
Volume31
Issue number32
DOIs
StatePublished - Nov 2010

Keywords

  • 3D culturing
  • Microchamber array
  • Multicellular breast cancer spheroids
  • Spheroid-based drug screening
  • UV adhesive microstructure

Fingerprint

Dive into the research topics of 'A polymer microstructure array for the formation, culturing, and high throughput drug screening of breast cancer spheroids'. Together they form a unique fingerprint.

Cite this