A newly designed whole microplate automatic harvester for lymphocyte stimulation assays

Jacob Dagan; Ilana Eshel; Ephraim Gazit; Moshe Plawner; Jacob Shoham

doi:10.1016/0022-1759(79)90277-1

A newly designed whole microplate automatic harvester for lymphocyte stimulation assays

Jacob Dagan, Ilana Eshel, Ephraim Gazit, Moshe Plawner, Jacob Shoham

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A unique automated sampling manifold designed to recover cells grown in standard 96 well microplates from their culture medium is described. Cells are recovered and washed on fiber glass filter discs. Incorporation of radioisotopes into cells, can then be measured by appropriate counting of the filter discs. Typical applications include termination of mixed lymphocyte cultures, assays of mitogen stimulation of lymphocytes and antigen-specific lymphocyte transformation and assays of interferon activity. The harvester can also be used in other biological systems where collection and washing of precipitates is desired.

Original language	English
Pages (from-to)	97-103
Number of pages	7
Journal	Journal of Immunological Methods
Volume	30
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(79)90277-1
State	Published - 19 Oct 1979
Externally published	Yes

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10.1016/0022-1759(79)90277-1

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abstract = "A unique automated sampling manifold designed to recover cells grown in standard 96 well microplates from their culture medium is described. Cells are recovered and washed on fiber glass filter discs. Incorporation of radioisotopes into cells, can then be measured by appropriate counting of the filter discs. Typical applications include termination of mixed lymphocyte cultures, assays of mitogen stimulation of lymphocytes and antigen-specific lymphocyte transformation and assays of interferon activity. The harvester can also be used in other biological systems where collection and washing of precipitates is desired.",

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AU - Eshel, Ilana

AU - Gazit, Ephraim

AU - Plawner, Moshe

AU - Shoham, Jacob

PY - 1979/10/19

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AB - A unique automated sampling manifold designed to recover cells grown in standard 96 well microplates from their culture medium is described. Cells are recovered and washed on fiber glass filter discs. Incorporation of radioisotopes into cells, can then be measured by appropriate counting of the filter discs. Typical applications include termination of mixed lymphocyte cultures, assays of mitogen stimulation of lymphocytes and antigen-specific lymphocyte transformation and assays of interferon activity. The harvester can also be used in other biological systems where collection and washing of precipitates is desired.

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A newly designed whole microplate automatic harvester for lymphocyte stimulation assays

Abstract

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