A new optical method for characterizing single molecule interactions based on dark field microscopy

Heidelinde R.C. Dietrich, Bart J. Vermolen, Bernd Rieger, Ian T. Young, Yuval Garini

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

5 Scopus citations

Abstract

Single-molecule techniques continue to gain in popularity in research disciplines such as the study of intermolecular interactions. These techniques provide information that otherwise would be lost by using bulk measurements that deal with a large number of molecules. We describe in this report the motion of tethered DNA molecules that have been tagged with gold nanobeads and observed under dark field microscopy to study single molecular interactions (SMI). We further report on the derivation and use of several physical parameters and how these parameters change under differing experimental conditions.

Original languageEnglish
Title of host publicationUltrasensitive and Single-Molecule Detection Technologies II
DOIs
StatePublished - 2007
Externally publishedYes
EventUltrasensitive and Single-Molecule Detection Technologies II - San Jose, CA, United States
Duration: 20 Jan 200723 Jan 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6444
ISSN (Print)1605-7422

Conference

ConferenceUltrasensitive and Single-Molecule Detection Technologies II
Country/TerritoryUnited States
CitySan Jose, CA
Period20/01/0723/01/07

Keywords

  • Dark field microscopy
  • Gold nanobeads
  • Molecular interactions
  • Single molecule detection
  • Tethered-particle motion

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