A monomeric 310-helix is formed in water by a 13-residue peptide representing the neutralizing determinant of HIV-1 on gp41

Zohar Biron, Sanjay Khare, Abraham O. Samson, Yehezkiel Hayek, Fred Naider, Jacob Anglister

Research output: Contribution to journalArticlepeer-review

95 Scopus citations

Abstract

The peptide gp41659-671 (ELLELDKWASLWN) comprises the entire epitope for one of the three known antibodies capable of neutralizing a broad spectrum of primary HIV-1 isolates and is the only such epitope that is sequential. Here we present the NMR structure of gp41659-671 in water. This peptide forms a monomeric 310-helix stabilized by i,i+3 side chain-side chain interactions favored by its primary sequence. In this conformation the peptide presents an exposed surface, which is mostly hydrophobic and consists of conserved HIV-1 residues. The presence of the 310-helix is confirmed by its characteristic CD pattern. Studies of the 310-helix have been hampered by the absence of a model peptide adopting this conformation, gp41659-671 can serve as such a model to investigate the spectral characteristics of the 310-helix, the factors that influence its stability, and the propensity of different amino acids to form a 310-helix. The observation that the 310-helical conformation is highly populated in the peptide gp41659-671 indicates that the corresponding segment in the cognate protein is an autonomous folding unit. As such, it is very likely that the helical conformation is maintained in gp41 throughout the different tertiary structures of the envelope protein that form during the process of viral fusion. However, the exposure of the gp41659-671 segment may vary, leading to changes in the reactivity of anti-gp41 antibodies in the different stages of viral fusion. Since gp41659-671 is an autonomous folding unit, peptide immunogens consisting of the complete gp41659-671 sequence are likely to induce antibodies highly cross-reactive with HIV-1.

Original languageEnglish
Pages (from-to)12687-12696
Number of pages10
JournalBiochemistry
Volume41
Issue number42
DOIs
StatePublished - 22 Oct 2002
Externally publishedYes

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