A microscope configuration for nanometer 3-D movement monitoring accuracy

Yevgeny Beiderman, Avigail D. Amsel, Yaniv Tzadka, Dror Fixler, Vicente Mico, Javier Garcia, Mina Teicher, Zeev Zalevsky

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

In this paper we present a new microscopy configuration based upon temporal tracking of a secondary reflected speckle by imaging the speckle through properly defocused optics. The configuration is used to monitor three-dimensional (3-D) spontaneous contraction of rat cardiac muscle cells while achieving nanometer tracking accuracy at a rate of 30 frames per second (fps) without using interferometric recording. Estimation of the change in the optical path of accuracy of 50. nm in the transverse direction and of 200. nm in the axial direction was achieved.

Original languageEnglish
Pages (from-to)366-375
Number of pages10
JournalMicron
Volume42
Issue number4
DOIs
StatePublished - Jun 2011

Keywords

  • Backscattering
  • Fourier optics
  • Movement monitoring
  • Speckle

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