A microscope configuration for nanometer 3-D movement monitoring accuracy

Yevgeny Beiderman; Avigail D. Amsel; Yaniv Tzadka; Dror Fixler; Vicente Mico; Javier Garcia; Mina Teicher; Zeev Zalevsky

doi:10.1016/j.micron.2010.05.020

A microscope configuration for nanometer 3-D movement monitoring accuracy

Yevgeny Beiderman, Avigail D. Amsel, Yaniv Tzadka, Dror Fixler, Vicente Mico, Javier Garcia, Mina Teicher, Zeev Zalevsky

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

In this paper we present a new microscopy configuration based upon temporal tracking of a secondary reflected speckle by imaging the speckle through properly defocused optics. The configuration is used to monitor three-dimensional (3-D) spontaneous contraction of rat cardiac muscle cells while achieving nanometer tracking accuracy at a rate of 30 frames per second (fps) without using interferometric recording. Estimation of the change in the optical path of accuracy of 50. nm in the transverse direction and of 200. nm in the axial direction was achieved.

Original language	English
Pages (from-to)	366-375
Number of pages	10
Journal	Micron
Volume	42
Issue number	4
DOIs	https://doi.org/10.1016/j.micron.2010.05.020
State	Published - Jun 2011

Keywords

Backscattering
Fourier optics
Movement monitoring
Speckle

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10.1016/j.micron.2010.05.020

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title = "A microscope configuration for nanometer 3-D movement monitoring accuracy",

abstract = "In this paper we present a new microscopy configuration based upon temporal tracking of a secondary reflected speckle by imaging the speckle through properly defocused optics. The configuration is used to monitor three-dimensional (3-D) spontaneous contraction of rat cardiac muscle cells while achieving nanometer tracking accuracy at a rate of 30 frames per second (fps) without using interferometric recording. Estimation of the change in the optical path of accuracy of 50. nm in the transverse direction and of 200. nm in the axial direction was achieved.",

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AU - Fixler, Dror

AU - Mico, Vicente

AU - Garcia, Javier

AU - Teicher, Mina

AU - Zalevsky, Zeev

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AB - In this paper we present a new microscopy configuration based upon temporal tracking of a secondary reflected speckle by imaging the speckle through properly defocused optics. The configuration is used to monitor three-dimensional (3-D) spontaneous contraction of rat cardiac muscle cells while achieving nanometer tracking accuracy at a rate of 30 frames per second (fps) without using interferometric recording. Estimation of the change in the optical path of accuracy of 50. nm in the transverse direction and of 200. nm in the axial direction was achieved.

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KW - Fourier optics

KW - Movement monitoring

KW - Speckle

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A microscope configuration for nanometer 3-D movement monitoring accuracy

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Keywords

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