A method to disentangle and quantify host anabolic turnover in photosymbiotic holobionts with subcellular resolution

Emma Gibbin, Guilhem Banc-Prandi, Maoz Fine, Arnaud Comment, Anders Meibom

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

A wide range of organisms host photosynthesizing symbionts. In these animals the metabolic exchange between host and symbionts has prevented in situ host anabolic turnover to be studied without the confounding effect of translocated photosynthates. Using the symbiotic coral Stylophora pistillata as a model organism and [1-13C]-pyruvate and [2,3-13C]-pyruvate in different incubation conditions (light, light + DCMU, and darkness), we employed NanoSIMS isotopic imaging to quantify host anabolism, with and without translocated metabolites from their photosynthesizing dinoflagellate symbionts. Under our experimental conditions, host de novo lipid synthesis accounted for ~40% of the total holobiont lipid reserve, and dinoflagellate recycling of metabolic 13CO2 enhanced host tissue 13C-enrichment by 13–22% in the epidermis, 40–58% in the gastrodermis, and 135–169% in host lipid bodies. Furthermore, we show that host anabolic turnover in different tissue structures differs, in a manner consistent with the localisation, function and cellular composition of these structures.

Original languageEnglish
Article number14
JournalCommunications Biology
Volume3
Issue number1
DOIs
StatePublished - 8 Jan 2020

Bibliographical note

Publisher Copyright:
© 2020, The Author(s).

Fingerprint

Dive into the research topics of 'A method to disentangle and quantify host anabolic turnover in photosymbiotic holobionts with subcellular resolution'. Together they form a unique fingerprint.

Cite this