A method for screening potential antioxidant activity

Toshikazu Nishida; Ritsuko Matsukawa; Kazuyoshi Masaki; Zvy Dubinsky; Isao Karube

doi:10.1016/0168-1656(96)01611-2

A method for screening potential antioxidant activity

Toshikazu Nishida, Ritsuko Matsukawa, Kazuyoshi Masaki, Zvy Dubinsky, Isao Karube

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A flow injection system for screening antioxidant activity was developed using lipoxygenase enzyme immobilized on 1,1'-carbonyldiimidazole activated cross-linked beaded agarose support. Oxygen consumption and hydroperoxide formation were measured using a Clark oxygen electrode and a UV detector located downstream relative to the flow column. The measurement of the rate of oxygen consumption and hydroperoxide formation provide a unique dual signal method to detect antioxidant activity. Antioxidant detection is demonstrated for microalgal extracts.

Original language	English
Pages (from-to)	149-155
Number of pages	7
Journal	Journal of Biotechnology
Volume	51
Issue number	2
DOIs	https://doi.org/10.1016/0168-1656(96)01611-2
State	Published - 1 Nov 1996

Keywords

Antioxidant activity
Flow injection
Immobilization
Lipoxygenase
Oxygen electrode
Screening

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10.1016/0168-1656(96)01611-2

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title = "A method for screening potential antioxidant activity",

abstract = "A flow injection system for screening antioxidant activity was developed using lipoxygenase enzyme immobilized on 1,1'-carbonyldiimidazole activated cross-linked beaded agarose support. Oxygen consumption and hydroperoxide formation were measured using a Clark oxygen electrode and a UV detector located downstream relative to the flow column. The measurement of the rate of oxygen consumption and hydroperoxide formation provide a unique dual signal method to detect antioxidant activity. Antioxidant detection is demonstrated for microalgal extracts.",

keywords = "Antioxidant activity, Flow injection, Immobilization, Lipoxygenase, Oxygen electrode, Screening",

author = "Toshikazu Nishida and Ritsuko Matsukawa and Kazuyoshi Masaki and Zvy Dubinsky and Isao Karube",

year = "1996",

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T1 - A method for screening potential antioxidant activity

AU - Nishida, Toshikazu

AU - Matsukawa, Ritsuko

AU - Masaki, Kazuyoshi

AU - Dubinsky, Zvy

AU - Karube, Isao

PY - 1996/11/1

Y1 - 1996/11/1

N2 - A flow injection system for screening antioxidant activity was developed using lipoxygenase enzyme immobilized on 1,1'-carbonyldiimidazole activated cross-linked beaded agarose support. Oxygen consumption and hydroperoxide formation were measured using a Clark oxygen electrode and a UV detector located downstream relative to the flow column. The measurement of the rate of oxygen consumption and hydroperoxide formation provide a unique dual signal method to detect antioxidant activity. Antioxidant detection is demonstrated for microalgal extracts.

AB - A flow injection system for screening antioxidant activity was developed using lipoxygenase enzyme immobilized on 1,1'-carbonyldiimidazole activated cross-linked beaded agarose support. Oxygen consumption and hydroperoxide formation were measured using a Clark oxygen electrode and a UV detector located downstream relative to the flow column. The measurement of the rate of oxygen consumption and hydroperoxide formation provide a unique dual signal method to detect antioxidant activity. Antioxidant detection is demonstrated for microalgal extracts.

KW - Antioxidant activity

KW - Flow injection

KW - Immobilization

KW - Lipoxygenase

KW - Oxygen electrode

KW - Screening

UR - https://www.scopus.com/pages/publications/0030297707

U2 - 10.1016/0168-1656(96)01611-2

DO - 10.1016/0168-1656(96)01611-2

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AN - SCOPUS:0030297707

SN - 0168-1656

VL - 51

SP - 149

EP - 155

JO - Journal of Biotechnology

JF - Journal of Biotechnology

IS - 2

ER -

A method for screening potential antioxidant activity

Abstract

Keywords

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