A logic-gated nanorobot for targeted transport of molecular payloads

Shawn M. Douglas, Ido Bachelet, George M. Church

Research output: Contribution to journalArticlepeer-review

1735 Scopus citations

Abstract

We describe an autonomous DNA nanorobot capable of transporting molecular payloads to cells, sensing cell surface inputs for conditional, triggered activation, and reconfiguring its structure for payload delivery. The device can be loaded with a variety of materials in a highly organized fashion and is controlled by an aptamer-encoded logic gate, enabling it to respond to a wide array of cues. We implemented several different logical AND gates and demonstrate their efficacy in selective regulation of nanorobot function. As a proof of principle, nanorobots loaded with combinations of antibody fragments were used in two different types of cell-signaling stimulation in tissue culture. Our prototype could inspire new designs with different selectivities and biologically active payloads for cell-targeting tasks.

Original languageEnglish
Pages (from-to)831-834
Number of pages4
JournalScience
Volume335
Issue number6070
DOIs
StatePublished - 17 Feb 2012
Externally publishedYes

Funding

FundersFunder number
Japan Society for the Promotion of Science23770216

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