Abstract
Induction of IFN-β1 RNA was studied in the mouse cell line SR117-21E transformed by a BPV episome containing the human IFN-β1 gene deleted of promoter sequences upstream from position -40. Nuclei isolated from these cells synthesize constitutively IFN-β1 RNA from the partially deleted promoter. The IFN-β1 RNA synthesized by nuclei of uninduced SR117-21E cells is similar to that made by nuclei of poly(rI):(rC)-induced cells, but does not accumulate and hence no IFN is produced unless the cells have been treated either by ds RNA or by cycloheximide. We conclude that the IFN-β1 gene has, in addition to the transcription control due to upstream promoter sequences, an additional post-transcriptional control acting on mRNA accumulation and linked to sequences close to the TATA box and RNA start site. Both controls are relieved by ds RNA.
| Original language | English |
|---|---|
| Pages (from-to) | 6979-6993 |
| Number of pages | 15 |
| Journal | Nucleic Acids Research |
| Volume | 12 |
| Issue number | 18 |
| DOIs | |
| State | Published - 25 Sep 1984 |
| Externally published | Yes |
Bibliographical note
Funding Information:ACKNOWLEDGEMENTS We thank Dr. S. Mitrani-Rosenbaum and P. Howley for communicating unpublished results. We thank Drs.Y. Mory, L. Maroteaux and Y. Groner for helpful discussions. Work supported in part by InterYeda, Israel.
Funding
ACKNOWLEDGEMENTS We thank Dr. S. Mitrani-Rosenbaum and P. Howley for communicating unpublished results. We thank Drs.Y. Mory, L. Maroteaux and Y. Groner for helpful discussions. Work supported in part by InterYeda, Israel.
| Funders | Funder number |
|---|---|
| InterYeda, Israel |