A disulfide conjugate between anti-tetanus antibodies and HIV (37-72)Tat neutralizes tetanus toxin inside chromaffin cells

Sylvia Stein; Aryeh Weiss; Knut Adermann; Philip Lazarovici; Jacob Hochman; Hans Wellhöner

doi:10.1016/s0014-5793(99)01186-2

A disulfide conjugate between anti-tetanus antibodies and HIV (37-72)Tat neutralizes tetanus toxin inside chromaffin cells

Sylvia Stein, Aryeh Weiss, Knut Adermann, Philip Lazarovici, Jacob Hochman, Hans Wellhöner

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Conjugates between anti-tetanus F(ab')₂ fragments and the (37-72) fragment of the HIV Tat protein were taken up by chromaffin cells, NG108-15 neurohybridoma cells and Rev-2-T-6 lymphoma cells. The uptake could not be inhibited by competition with (37-72)Tat, but was reduced in the presence of metabolic inhibitors or at low temperature. The disulfide as well as the thioether conjugate were translocated to the cytoplasmic space, but only the disulfide conjugate moderately restored the stimulated transmitter release inhibited by tetanus toxin. Therefore, disulfide conjugates are more promising than thioethers for the neutralization of intracellular antigens. These conjugates provide new tools to study neuroprotection against bacterial neurotoxins. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	383-386
Number of pages	4
Journal	FEBS Letters
Volume	458
Issue number	3
DOIs	https://doi.org/10.1016/s0014-5793(99)01186-2
State	Published - 24 Sep 1999
Externally published	Yes

Bibliographical note

Funding Information:
We would like to thank Mrs. Carola Kassebaum for valuable technical assistance. This work was supported in part by a grant from the Lower Saxony-Israel Research Fund to J.H., P.L., and H.W. Dr. Philip Lazarovici is affiliated with the David R. Bloom Center at the Hebrew University.

Funding

We would like to thank Mrs. Carola Kassebaum for valuable technical assistance. This work was supported in part by a grant from the Lower Saxony-Israel Research Fund to J.H., P.L., and H.W. Dr. Philip Lazarovici is affiliated with the David R. Bloom Center at the Hebrew University.

Funders	Funder number
Lower Saxony-Israel Research Fund
Hebrew University of Jerusalem

Keywords

Disulfide conjugate
HIV-Tat
Tetanus toxin
Thioether conjugate

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/s0014-5793(99)01186-2

Cite this

@article{83953f3ce4c94ae4b988e73dd9c96748,

title = "A disulfide conjugate between anti-tetanus antibodies and HIV (37-72)Tat neutralizes tetanus toxin inside chromaffin cells",

abstract = "Conjugates between anti-tetanus F(ab')2 fragments and the (37-72) fragment of the HIV Tat protein were taken up by chromaffin cells, NG108-15 neurohybridoma cells and Rev-2-T-6 lymphoma cells. The uptake could not be inhibited by competition with (37-72)Tat, but was reduced in the presence of metabolic inhibitors or at low temperature. The disulfide as well as the thioether conjugate were translocated to the cytoplasmic space, but only the disulfide conjugate moderately restored the stimulated transmitter release inhibited by tetanus toxin. Therefore, disulfide conjugates are more promising than thioethers for the neutralization of intracellular antigens. These conjugates provide new tools to study neuroprotection against bacterial neurotoxins. Copyright (C) 1999 Federation of European Biochemical Societies.",

keywords = "Disulfide conjugate, HIV-Tat, Tetanus toxin, Thioether conjugate",

author = "Sylvia Stein and Aryeh Weiss and Knut Adermann and Philip Lazarovici and Jacob Hochman and Hans Wellh{\"o}ner",

note = "Funding Information: We would like to thank Mrs. Carola Kassebaum for valuable technical assistance. This work was supported in part by a grant from the Lower Saxony-Israel Research Fund to J.H., P.L., and H.W. Dr. Philip Lazarovici is affiliated with the David R. Bloom Center at the Hebrew University. ",

year = "1999",

month = sep,

day = "24",

doi = "10.1016/s0014-5793(99)01186-2",

language = "אנגלית",

volume = "458",

pages = "383--386",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - A disulfide conjugate between anti-tetanus antibodies and HIV (37-72)Tat neutralizes tetanus toxin inside chromaffin cells

AU - Stein, Sylvia

AU - Weiss, Aryeh

AU - Adermann, Knut

AU - Lazarovici, Philip

AU - Hochman, Jacob

AU - Wellhöner, Hans

N1 - Funding Information: We would like to thank Mrs. Carola Kassebaum for valuable technical assistance. This work was supported in part by a grant from the Lower Saxony-Israel Research Fund to J.H., P.L., and H.W. Dr. Philip Lazarovici is affiliated with the David R. Bloom Center at the Hebrew University.

PY - 1999/9/24

Y1 - 1999/9/24

N2 - Conjugates between anti-tetanus F(ab')2 fragments and the (37-72) fragment of the HIV Tat protein were taken up by chromaffin cells, NG108-15 neurohybridoma cells and Rev-2-T-6 lymphoma cells. The uptake could not be inhibited by competition with (37-72)Tat, but was reduced in the presence of metabolic inhibitors or at low temperature. The disulfide as well as the thioether conjugate were translocated to the cytoplasmic space, but only the disulfide conjugate moderately restored the stimulated transmitter release inhibited by tetanus toxin. Therefore, disulfide conjugates are more promising than thioethers for the neutralization of intracellular antigens. These conjugates provide new tools to study neuroprotection against bacterial neurotoxins. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - Conjugates between anti-tetanus F(ab')2 fragments and the (37-72) fragment of the HIV Tat protein were taken up by chromaffin cells, NG108-15 neurohybridoma cells and Rev-2-T-6 lymphoma cells. The uptake could not be inhibited by competition with (37-72)Tat, but was reduced in the presence of metabolic inhibitors or at low temperature. The disulfide as well as the thioether conjugate were translocated to the cytoplasmic space, but only the disulfide conjugate moderately restored the stimulated transmitter release inhibited by tetanus toxin. Therefore, disulfide conjugates are more promising than thioethers for the neutralization of intracellular antigens. These conjugates provide new tools to study neuroprotection against bacterial neurotoxins. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - Disulfide conjugate

KW - HIV-Tat

KW - Tetanus toxin

KW - Thioether conjugate

UR - http://www.scopus.com/inward/record.url?scp=0032883128&partnerID=8YFLogxK

U2 - 10.1016/s0014-5793(99)01186-2

DO - 10.1016/s0014-5793(99)01186-2

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 10570945

AN - SCOPUS:0032883128

SN - 0014-5793

VL - 458

SP - 383

EP - 386

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

A disulfide conjugate between anti-tetanus antibodies and HIV (37-72)Tat neutralizes tetanus toxin inside chromaffin cells

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this