Abstract
Key message: Overview of pollen transcriptome studies. Pollen development is driven by gene expression, and knowledge of the molecular events underlying this process has undergone a quantum leap in the last decade through studies of the transcriptome. Here, we outline historical evidence for male haploid gene expression and review the wealth of pollen transcriptome data now available. Knowledge of the transcriptional capacity of pollen has progressed from genetic studies to the direct analysis of RNA and from gene-by-gene studies to analyses on a genomic scale. Microarray and/or RNA-seq data can now be accessed for all phases and cell types of developing pollen encompassing 10 different angiosperms. These growing resources have accelerated research and will undoubtedly inspire new directions and the application of system-based research into the mechanisms that govern the development, function and evolution of angiosperm pollen.
| Original language | English |
|---|---|
| Pages (from-to) | 73-89 |
| Number of pages | 17 |
| Journal | Plant Reproduction |
| Volume | 28 |
| Issue number | 2 |
| DOIs | |
| State | Published - 16 Jun 2015 |
| Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2015, The Author(s).
Funding
We thank the Biotechnology and Biological Science Research Council (BBSRC) for grant support and for the award of a studentship to NR. We also thank Matt Blades from the Bioinformatics and Biostatistics Analysis Support Hub (BBASH) for help with PCA, Ann Loraine for helpful discussions on Arabidopsis and tomato pollen RNA-seq data, and Jerome Twell for help in drafting Figs. and .
| Funders | Funder number |
|---|---|
| Biotechnology and Biological Sciences Research Council |
Keywords
- Development
- Gene expression
- Male gametophyte
- Pollen
- Transcriptome