TY - JOUR
T1 - A cyanobacterial gene encoding peptidyl-prolyl cis-trans isomerase
AU - Hassidim, Miriam
AU - Schwarz, Rakefet
AU - Lieman-Hurwitz, Judy
AU - Marco, Eduardo
AU - Ronen-Tarazi, Michal
AU - Kaplan, Aaron
PY - 1992/12
Y1 - 1992/12
N2 - The rotA gene encoding peptidyl-prolyl cis-trans isomerase has been identified, sequenced, and shown to be transcribed in the cyanobacterium Synechococcus sp. PCC7942. Inactivation of the gene by replacement of a region containing the open reading frame with a gene conferring kanamycin resistance resulted in merodiploids containing both the wild type and the modified genomic region. We were not able to isolate a kanamycin-resistant mutant in which all the genomic wild-type copies were substituted, which suggests that such replacement could have been lethal.
AB - The rotA gene encoding peptidyl-prolyl cis-trans isomerase has been identified, sequenced, and shown to be transcribed in the cyanobacterium Synechococcus sp. PCC7942. Inactivation of the gene by replacement of a region containing the open reading frame with a gene conferring kanamycin resistance resulted in merodiploids containing both the wild type and the modified genomic region. We were not able to isolate a kanamycin-resistant mutant in which all the genomic wild-type copies were substituted, which suggests that such replacement could have been lethal.
UR - http://www.scopus.com/inward/record.url?scp=0000205828&partnerID=8YFLogxK
U2 - 10.1104/pp.100.4.1982
DO - 10.1104/pp.100.4.1982
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AN - SCOPUS:0000205828
SN - 0032-0889
VL - 100
SP - 1982
EP - 1986
JO - Plant Physiology
JF - Plant Physiology
IS - 4
ER -