A comparison of screening methods for antioxidant activity in seaweeds

R. Matsukawa, Z. Dubinsky, E. Kishimoto, K. Masaki, Y. Masuda, T. Takeuchi, M. Chihara, Y. Yamamoto, E. Niki, I. Karube

    Research output: Contribution to journalArticlepeer-review

    222 Scopus citations


    The inhibition of lipid peroxidation and radical scavenging effects were studied to evaluate the antioxidant activity for extracts of 17 species of seaweed. The antioxidant effect was evaluated by determination of lipoxygenase activity and by α, α-diphenyl-β-picrylhydrazyl (DPPH) decolorization. Lipoxygenase activity was depressed in the presence of aqueous and ethanol extracts of 4 algal species; Sargassum species had the highest antioxidant activity of all the species examined. The ethanol extracts of one Sargassum species showed competitive inhibition with the substrate. The same species also showed radical scavenging activity in the DPPH decolorization test. Comparison of these results shows no relationship between enzyme inhibition and radical scavenging activity.

    Original languageEnglish
    Pages (from-to)29-35
    Number of pages7
    JournalJournal of Applied Phycology
    Issue number1
    StatePublished - 1997

    Bibliographical note

    Funding Information:
    This work was supported by Toyo Suisan Kaisha, Japan. We would like to thank Dr Scott McNiven for linguistic assistance.


    • Antioxidant activity
    • Lipoxygenase inhibition
    • Radical scavenging activity
    • Screening
    • Seaweed


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