A comparison of screening methods for antioxidant activity in seaweeds

R. Matsukawa; Z. Dubinsky; E. Kishimoto; K. Masaki; Y. Masuda; T. Takeuchi; M. Chihara; Y. Yamamoto; E. Niki; I. Karube

doi:10.1023/A:1007935218120

A comparison of screening methods for antioxidant activity in seaweeds

R. Matsukawa, Z. Dubinsky, E. Kishimoto, K. Masaki, Y. Masuda, T. Takeuchi, M. Chihara, Y. Yamamoto, E. Niki, I. Karube

Department of Geography

Research output: Contribution to journal › Article › peer-review

157 Scopus citations

Abstract

The inhibition of lipid peroxidation and radical scavenging effects were studied to evaluate the antioxidant activity for extracts of 17 species of seaweed. The antioxidant effect was evaluated by determination of lipoxygenase activity and by α, α-diphenyl-β-picrylhydrazyl (DPPH) decolorization. Lipoxygenase activity was depressed in the presence of aqueous and ethanol extracts of 4 algal species; Sargassum species had the highest antioxidant activity of all the species examined. The ethanol extracts of one Sargassum species showed competitive inhibition with the substrate. The same species also showed radical scavenging activity in the DPPH decolorization test. Comparison of these results shows no relationship between enzyme inhibition and radical scavenging activity.

Original language	English
Pages (from-to)	29-35
Number of pages	7
Journal	Journal of Applied Phycology
Volume	9
Issue number	1
DOIs	https://doi.org/10.1023/A:1007935218120
State	Published - 1997

Bibliographical note

Funding Information:
This work was supported by Toyo Suisan Kaisha, Japan. We would like to thank Dr Scott McNiven for linguistic assistance.

Keywords

Antioxidant activity
Lipoxygenase inhibition
Radical scavenging activity
Screening
Seaweed

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10.1023/A:1007935218120

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title = "A comparison of screening methods for antioxidant activity in seaweeds",

abstract = "The inhibition of lipid peroxidation and radical scavenging effects were studied to evaluate the antioxidant activity for extracts of 17 species of seaweed. The antioxidant effect was evaluated by determination of lipoxygenase activity and by α, α-diphenyl-β-picrylhydrazyl (DPPH) decolorization. Lipoxygenase activity was depressed in the presence of aqueous and ethanol extracts of 4 algal species; Sargassum species had the highest antioxidant activity of all the species examined. The ethanol extracts of one Sargassum species showed competitive inhibition with the substrate. The same species also showed radical scavenging activity in the DPPH decolorization test. Comparison of these results shows no relationship between enzyme inhibition and radical scavenging activity.",

keywords = "Antioxidant activity, Lipoxygenase inhibition, Radical scavenging activity, Screening, Seaweed",

author = "R. Matsukawa and Z. Dubinsky and E. Kishimoto and K. Masaki and Y. Masuda and T. Takeuchi and M. Chihara and Y. Yamamoto and E. Niki and I. Karube",

note = "Funding Information: This work was supported by Toyo Suisan Kaisha, Japan. We would like to thank Dr Scott McNiven for linguistic assistance.",

year = "1997",

doi = "10.1023/A:1007935218120",

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AU - Matsukawa, R.

AU - Dubinsky, Z.

AU - Kishimoto, E.

AU - Masaki, K.

AU - Masuda, Y.

AU - Takeuchi, T.

AU - Chihara, M.

AU - Yamamoto, Y.

AU - Niki, E.

AU - Karube, I.

N1 - Funding Information: This work was supported by Toyo Suisan Kaisha, Japan. We would like to thank Dr Scott McNiven for linguistic assistance.

PY - 1997

Y1 - 1997

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AB - The inhibition of lipid peroxidation and radical scavenging effects were studied to evaluate the antioxidant activity for extracts of 17 species of seaweed. The antioxidant effect was evaluated by determination of lipoxygenase activity and by α, α-diphenyl-β-picrylhydrazyl (DPPH) decolorization. Lipoxygenase activity was depressed in the presence of aqueous and ethanol extracts of 4 algal species; Sargassum species had the highest antioxidant activity of all the species examined. The ethanol extracts of one Sargassum species showed competitive inhibition with the substrate. The same species also showed radical scavenging activity in the DPPH decolorization test. Comparison of these results shows no relationship between enzyme inhibition and radical scavenging activity.

KW - Antioxidant activity

KW - Lipoxygenase inhibition

KW - Radical scavenging activity

KW - Screening

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A comparison of screening methods for antioxidant activity in seaweeds

Abstract

Bibliographical note

Keywords

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