A cell free system reveals that capacitation is a prerequisite for membrane fusion during the acrosome reaction

Ben Spungin; Tova Levinshal; Sara Rubinstein; Haim Breitbart

doi:10.1016/0014-5793(92)81388-3

A cell free system reveals that capacitation is a prerequisite for membrane fusion during the acrosome reaction

Ben Spungin, Tova Levinshal, Sara Rubinstein, Haim Breitbart

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Plasma and outer acrosomal membranes were extracted from bovine spermatozoa and used in an in vitro fusion assay. Fusion was revealed by monitoring the merging of lipids using the chlorophyll a-N,N′-dioctadecyloxacarbocyanine-p-toluene sulfonate (DCY) method [(1984) Biochim. Biophys. Acta 769. 531-542]. The requirement for capacitation, as well as the effects or pH, calcium and spermine, on membrane fusion in our cell-free system were similar to those observed in vivo on the acrosomal reaction. This demonstrates for the first time that capacitation and alterations in intracellular pH and calcium concentration, which must precede the acrosomal reaction, are required for the membrane fusion event.

Original language	English
Pages (from-to)	155-160
Number of pages	6
Journal	FEBS Letters
Volume	311
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(92)81388-3
State	Published - 19 Oct 1992

Keywords

Acrosomal reaction
Exocytosis
Fertilization
Membrane fusion
Secretion
Sperm

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10.1016/0014-5793(92)81388-3

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abstract = "Plasma and outer acrosomal membranes were extracted from bovine spermatozoa and used in an in vitro fusion assay. Fusion was revealed by monitoring the merging of lipids using the chlorophyll a-N,N′-dioctadecyloxacarbocyanine-p-toluene sulfonate (DCY) method [(1984) Biochim. Biophys. Acta 769. 531-542]. The requirement for capacitation, as well as the effects or pH, calcium and spermine, on membrane fusion in our cell-free system were similar to those observed in vivo on the acrosomal reaction. This demonstrates for the first time that capacitation and alterations in intracellular pH and calcium concentration, which must precede the acrosomal reaction, are required for the membrane fusion event.",

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AU - Breitbart, Haim

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A cell free system reveals that capacitation is a prerequisite for membrane fusion during the acrosome reaction

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Keywords

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