TY - JOUR
T1 - A 28,000 mol. wt toxin from Bacillus thuringiensis israelensis induces cation transport in rat muscle cultures
AU - Cahan, R.
AU - Shainberg, A.
AU - Pechatnikov, I.
AU - Nitzan, Y.
N1 - Funding Information:
Acknowledgemenrs-This study was supportedin part by the Health SciencesR esearchC enterf unds (to YN), and by The Otto Myerhoff Drug ReceptorC enterfunds (to AS).
PY - 1995/7
Y1 - 1995/7
N2 - The mechanism by which the Bacillus thuringiensis israelensis (Bti) 28,000 mol. wt toxin exerts its effect on mature muscle cultures was examined. The toxin inhibited Na+ K+-ATPase activity as revealed by 86Rb influx. A 50% inhibition of Na+ K+-ATPase activity was obtained with 0.2 μg/ml of the toxin. The inhibition was time and dose dependent, and it was reversible with low doses of the toxin (up to 0.2 μg/ml. A considerable release of 86Rb was obtained by doses greater than 0.2 μg/ml. The 86Rb release was also time and dose dependent. This effect is probably non-specific, since 45Ca influx is also accelerated by toxin-treated cultures. Pre-incubation of the toxin with phosphotidylserine (PS) antagonized the toxin. It is concluded that the toxin is a hydrophobic protein which interacts with the membrane. In low doses this interaction reduces the activity of the sodium pump and in high doses it causes non-specific permeability of the sarcolemma.
AB - The mechanism by which the Bacillus thuringiensis israelensis (Bti) 28,000 mol. wt toxin exerts its effect on mature muscle cultures was examined. The toxin inhibited Na+ K+-ATPase activity as revealed by 86Rb influx. A 50% inhibition of Na+ K+-ATPase activity was obtained with 0.2 μg/ml of the toxin. The inhibition was time and dose dependent, and it was reversible with low doses of the toxin (up to 0.2 μg/ml. A considerable release of 86Rb was obtained by doses greater than 0.2 μg/ml. The 86Rb release was also time and dose dependent. This effect is probably non-specific, since 45Ca influx is also accelerated by toxin-treated cultures. Pre-incubation of the toxin with phosphotidylserine (PS) antagonized the toxin. It is concluded that the toxin is a hydrophobic protein which interacts with the membrane. In low doses this interaction reduces the activity of the sodium pump and in high doses it causes non-specific permeability of the sarcolemma.
UR - http://www.scopus.com/inward/record.url?scp=0029058793&partnerID=8YFLogxK
U2 - 10.1016/0041-0101(95)00007-9
DO - 10.1016/0041-0101(95)00007-9
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C2 - 8588218
AN - SCOPUS:0029058793
SN - 0041-0101
VL - 33
SP - 943
EP - 951
JO - Toxicon
JF - Toxicon
IS - 7
ER -