Introduction:
The Leica 3D STED (Stimulated Emission Depletion Microscopy) system achieves resolution below the diffraction limit in both the lateral, and axial directions. Our Leica SP8 offers one of the only tunable white light lasers in Israel. There are two STED continuous wave lasers at 592 nm and 660 nm which allow the use of fluorophores up to 595nm in super-resolution mode.
Overview:
The SP8 has been designed for confocal microscopy with optimal photon efficiency and high speed. All optical components are matched towards preserving fluorescence photons for image contrast and to improve cell viability in live cell imaging.
Specifications:
Inverted Leica DMi8 with anti-vibration table
Z-Galvanometer
Continuous wave laser: UV- Diode 50mW 405nm
Pulsed laser: White Light Laser (WLL). power 1.5mW:470 - 670nm
Depletion lasers: two continuous (CW) wave lasers at 592 nm and 660 nm
Spectral Detection Range of 400nm - 720nm
Detectors: two HyD (hybrid detectors), two PMTs, and a TLD (transmitted light detector)
Excitation Modulation: AOTF: up to 8 channels
Resonant Scanner 8kHz (Maximal Frame Resolution: 1024x1024 pixel; Scan-Zoom: 1.3 - 48x. Up to 7 FPS at 512x512, up to 84 FPS at 512x16)
Accelerated Z-stacking with the SuperZ galvanometer substage
Name | Leica SP8 STED (2015) |
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Manufacturers | Leica Microsystems |
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