High Content Screening

Opera Phenix® Plus is a high throughput microplate confocal imager for High-Content Screening (HCS). It can acquire, analyze and manage fluorescence, brightfield and digital phase contrast images.


Configuration

Two sCMOS cameras:
7 mega pixel (2160x2160), 16 bit resolution, 6.5 μm pixel size
Simultaneous acquisition of up to four channels with minimized crosstalk
Each camera supports fast recording mode with up to 100 fps
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Proprietary Synchrony Optics combine a microlens-enhanced Nipkow spinning disk with dual view confocal optics to separate fluorescence excitation and emission during simultaneous aquisition
Excitation Configurations with 5 lasers - 375 nm 50mW, 425 nm 100mW, 488 nm 150mW, 561 nm 100mW, 640 nm 140mW.
Imaging Modality Selectable widefield and confocal imaging modes depending on the assay requirements
Microlens-enhanced dual spinning disk confocal unit with optimized pinhole-to-pinhole distance to further reduce out-of-focus noise in thick samples such as spheroids
Transmission brightfield and digital phase contrast
Emission Filter 435-515 nm, Typically used for CFP/YFP FRET studies
Air Objective:
5x Air objectiveNA 0.16, WD 12.1 mm
10x Air objective NA 0.3, WD 5.2 mm
20x Air, NA 0.4, WD 8.28 mm
Water immersion objectives – provides an improved light collection efficiency:
20x Water Immersion Objective NA 1.0, WD 1.7 mm
40x Water Immersion Objective NA 1.1, WD 0.62 mm
63x Water Immersion Objective NA 1.15, WD 0.6 mm
Sample chamber with temperature, CO2 and humidity control to enable live cell experiments.
Compatible with various well plates – 96, 384 etc.
Slide Holder (up to 4 slides) x 4