Cell Sorter - BD FACSAria-III with 5 lasers

  • Hagit Hauschner (Manager)

Equipment/facility: Equipment

Equipments Details

Description

BD FACSAria-III cell sorter is a digital sorter enabling high-speed sorting and multicolor analysis.
The instrument accommodates 4 excitation lasers at 405, 488, 561, 633 -nm and 15 detectors. It has also a 445nm Laser that can be use instead of the 488nm laser. This allows better detection of Cyan Fluorescent Protein (CFP). Acquisition rates can go up to 70,000 events/second.

Sorting options:
Two-way sorting into 15mL tubes. Four-way sorting into microtubes and 12 X 75 tubes. Including cooling apparatus. ACDU (automated cell deposition unit) for sorting into multi-well plates and onto microscope slides. Including single cell sorting.

Nozzle selection:
A set of four nozzles allows users sort a wide range of particle sizes and sort in low pressure for high recovery or in high pressure for high speed. Available nozzle sizes: 70, 85, 100, and 130 microns.

Sterile sorting:
Preparation for aseptic sort can be activated for one hour before the sort. Users who wish to run aseptic sort should prepare 10L sterile PBS and 2L sterile DDW.

Working policy:
High skill is required for working with the sorter. User who works often with the machine can undergo training and thereafter work independently. All others should contact Dr. Hagit Hauschner before scheduling.

Details

NameBD FACSAria-III cell sorter
ManufacturersBecton, Dickinson and Company (BD) Biosciences

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